Article
Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulence.
Molecular microbiology - 1 May 1990
Barny M A, Guinebretière M H, Marçais B, Coissac E, Paulin J P, Laurent J
Abstract excerpt
Phage MudIIPR13 insertional mutagenesis of Erwinia amylovora CFBP1430 allowed us to isolate 6900 independent CmR mutants. The frequencies of different auxotrophs in this population indicated that MudIIPR13 had inserted randomly in E. amylovora. Screening of 3500 CmR mutants on (i) apple calli and (ii) pear and apple seedlings led to the isolation of 19 non-pathogenic prototrophic single mutants, four of which...
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