Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulence.

Molecular microbiology - 1 May 1990

Barny M A, Guinebretière M H, Marçais B, Coissac E, Paulin J P, Laurent J

Abstract excerpt

Phage MudIIPR13 insertional mutagenesis of Erwinia amylovora CFBP1430 allowed us to isolate 6900 independent CmR mutants. The frequencies of different auxotrophs in this population indicated that MudIIPR13 had inserted randomly in E. amylovora. Screening of 3500 CmR mutants on (i) apple calli and (ii) pear and apple seedlings led to the isolation of 19 non-pathogenic prototrophic single mutants, four of which...

Topics

Cloning, Molecular
Cosmids
Erwinia
Genetic Complementation Test
Lac Operon
Multigene Family
Mutation
Plants, Toxic
Pseudomonas
Recombinant Fusion Proteins
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nicotiana

Virulence

beta-Galactosidase

Source

Identifiers

PMID: 2117695
DOI: 10.1111/j.1365-2958.1990.tb00648.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2117695-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulence.PMID 2117695Read ↗

Bacteriophage Mu as a genetic tool to study Erwinia amylovora pathogenicity and hypersensitive reaction on tobacco.Semantic proximity Read ↗

Gene for pathogenicity and ability to cause the hypersensitive reaction cloned from Erwinia amylovora.Semantic proximity Read ↗

Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host.Semantic proximity Read ↗

Virulence Genetics of an Erwinia amylovora Putative Polysaccharide Transporter Family Member.Semantic proximity Read ↗

Mutations in ams genes of Erwinia amylovora affect the interactions with host plants.Semantic proximity Read ↗

Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization.Semantic proximity Read ↗

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.Semantic proximity Read ↗

The bacterial alarmone (p)ppGpp activates the type III secretion system in Erwinia amylovora.Semantic proximity Read ↗

The fire blight pathogen Erwinia amylovora requires the rpoN gene for pathogenicity in apple.Semantic proximity Read ↗

The presence of hrp genes on the pathogenicity-associated plasmid of the tumorigenic bacterium Erwinia herbicola pv. gypsophilae.Semantic proximity Read ↗

Molecular signature of differential virulence in natural isolates of Erwinia amylovora.Semantic proximity Read ↗

Erwinia chrysanthemi hrp genes and their involvement in soft rot pathogenesis and elicitation of the hypersensitive response.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.