Article
Rectal swab DNA collection protocol for PCR genotyping in rats.
BioTechniques - 1 Jan 2024
Kaye Audrey E, Proctor-Bonbright Jacob W, Yu Jai Y
Abstract excerpt
DNA collection is essential for genotyping laboratory animals. Common collection methods require tissue amputation, causing discomfort and injury. Rectal swabbing has been proposed as an effective, minimally invasive alternative, but an evidence-backed protocol for the technique remains unavailable. This report evaluates the effects of collection parameters on the quality of PCR results and presents a protocol...
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