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Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases.

Macromolecular bioscience - 23 Feb 2005

Tsuge Takeharu, Yano Kotaro, Imazu Shin-ichi, Numata Keiji, Kikkawa Yoshihiro, Abe Hideki, Taguchi Seiichi, Doi Yoshiharu

Abstract excerpt

Eleven laboratory-evolved polyhydroxyalkanoate (PHA) synthases which originated from Pseudomonas sp. 61-3 enzyme (PhaC1(Ps)), together with the wild-type enzyme, were applied for PHA synthesis from fructose using Ralstonia eutropha PHB(-)4 as a host strain. The evolved PhaC1(Ps) mutants had amino acid substitution(s) at position 325 and/or position 481. In these mutants, serine-325 (S325) was replaced by cysteine...

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PMID: 15729719
DOI: 10.1002/mabi.200400152

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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