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Article

Probing the Ca(2+) switch of the neuronal Ca(2+) sensor GCAP2 by time-resolved fluorescence spectroscopy.

ACS chemical biology - 15 Jun 2012

Kollmann Heiko, Becker Simon F, Shirdel Javid, Scholten Alexander, Ostendorp Anna, Lienau Christoph, Koch Karl-Wilhelm

Abstract excerpt

We report fluorescence lifetime and rotational anisotropy measurements of the fluorescent dye Alexa647 attached to the guanylate cyclase-activating protein 2 (GCAP2), an intracellular myristoylated calcium sensor protein operating in photoreceptor cells. By linking the dye to different protein regions critical for monitoring calcium-induced conformational changes, we could measure fluorescence lifetimes and...

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PMID: 22409623
DOI: 10.1021/cb3000748

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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