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Probing the Ca(2+) switch of the neuronal Ca(2+) sensor GCAP2 by time-resolved fluorescence spectroscopy.

ACS chemical biology - 15 Jun 2012

Kollmann Heiko, Becker Simon F, Shirdel Javid, Scholten Alexander, Ostendorp Anna, Lienau Christoph, Koch Karl-Wilhelm

Abstract excerpt

We report fluorescence lifetime and rotational anisotropy measurements of the fluorescent dye Alexa647 attached to the guanylate cyclase-activating protein 2 (GCAP2), an intracellular myristoylated calcium sensor protein operating in photoreceptor cells. By linking the dye to different protein regions critical for monitoring calcium-induced conformational changes, we could measure fluorescence lifetimes and...

Topics

Animals
Calcium
Carbocyanines
Cattle
Escherichia coli
Fluorescence Polarization
Fluorescent Dyes
Gene Expression
Guanylate Cyclase-Activating Proteins
Models, Molecular
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Photoreceptor Cells

Protein Binding

Protein Conformation

Spectrometry, Fluorescence

Source

Identifiers

PMID: 22409623
DOI: 10.1021/cb3000748

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