Article
Alterations in specific and general protein synthesis after heat shock in heat-sensitive mutants of CHO cells and their wild-type counterparts.
Radiation research - 1 Oct 1990
Harvey W F, Bedford J S, Li G C
Abstract excerpt
The rates of general and specific protein synthesis were studied in two heat-sensitive strains of CHO cells (Harvey and Bedford, Radiat. Res. 113, 526-542, 1988), both of which show a reduced ability to develop thermotolerance following an initial 45 degrees C heat shock. After various labeling periods with [35S]methionine, wild-type and mutant labeled proteins were separated by one- and two-dimensional...
Topics
- Acclimatization
- Animals
- Autoradiography
- Cell Line
- Cricetinae
- Cricetulus
- Electrophoresis, Gel, Two-Dimensional
- Heat-Shock Proteins
- Hot Temperature
- In Vitro Techniques
- Mutation
