Article
Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Endo Masaki, Shimizu Tsutomu, Toki Seiichi
Abstract excerpt
Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice ALS [OsmALS (W548L/S627I)] confers resistance to BS. We succeeded in using OsmALS with native OsALS promoter and terminator region for a selection marker of rice...
Topics
- Acetolactate Synthase
- Benzoates
- Herbicide Resistance
- Herbicides
- Mutation
- Oryza
- Plants, Genetically Modified
- Promoter Regions, Genetic
- Pyrimidines
