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Article

Mutant acetolactate synthase gene is an efficient in vitro selectable marker for the genetic transformation of Brassica juncea (oilseed mustard).

Journal of plant physiology - 1 Sept 2004

Ray Krishna, Jagannath Arun, Gangwani Suveena Arora, Burma Pradeep Kumar, Pental Deepak

Abstract excerpt

We report in this study, the successful deployment of a double mutant acetolactate synthase gene (ALSdm, containing Pro 197 to Ser and Ser 653 to Asn substitutions) as an efficient in vitro selection marker for the development of transgenic plants in Brassica juncea (oilseed mustard). The ALS enzyme is inhibited by two categories of herbicides, sulfonylureas (e.g. chlorsulfuron) and imidazolinones (e.g....

Topics

Acetolactate Synthase
Drug Resistance
Genetic Markers
Herbicides
Kanamycin
Mustard Plant
Mutation
Nicotinic Acids
Plants, Genetically Modified
Sulfonamides
Transformation, Genetic

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Identifiers

PMID: 15499910
DOI: 10.1016/j.jplph.2004.02.001

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15499910-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Mutant acetolactate synthase gene is an efficient in vitro selectable marker for the genetic transformation of Brassica juncea (oilseed mustard).PMID 15499910Read ↗

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