Article
Molecular characterization of true and ectopic gene targeting events at the acetolactate synthase gene in Arabidopsis.
Plant & cell physiology - 1 Mar 2006
Endo Masaki, Osakabe Keishi, Ichikawa Hiroaki, Toki Seiichi
Abstract excerpt
Precise modification of plant genomes via gene targeting (GT) is important for the study of gene function in vivo. A reliable GT system using the protoporphyrinogen oxidase (PPO) gene in Arabidopsis was reported 4 years ago; however, there are no subsequent successful reports of GT in Arabidopsis. A previous study showed ectopic gene targeting (EGT) of the endogenous gene in two-thirds of GT plants, which was an...
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