Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker.

doi:10.1007/s00299-008-0553-6

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Article

Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker.

Plant cell reports - 1 Aug 2008

Ogawa Taiichi, Kawahigashi Hiroyuki, Toki Seiichi, Handa Hirokazu

Abstract excerpt

Acetolactate synthase (ALS) is a target enzyme for many herbicides, including sulfonylurea and imidazolinone. We investigated the usefulness of a mutated ALS gene of rice, which had double point mutations and encoded an herbicide-resistant form of the enzyme, as a selectable marker for wheat tran...

Topics

Acetolactate Synthase
Blotting, Southern
Herbicide Resistance
Herbicides
Mutation
Oryza
Plant Proteins
Plants, Genetically Modified
Polymerase Chain Reaction
Transformation, Genetic
Triticum

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