Article
Characterization of clones of HIV-1 infected HuT 78 cells defective in gag gene processing and of SIV clones producing large amounts of envelope glycoprotein.
Journal of medical primatology - 1 Jan 1990
Benveniste R E, Hill R W, Eron L J, Csaikl U M, Knott W B, Henderson L E, Sowder R C, Nagashima K, Gonda M A
Abstract excerpt
Single-cell clones of HIV-1 (FRE-3) or SIV/Mne infected HuT 78 cells were obtained by plating dilutions of virally infected HuT 78 cells on a monolayer of sheep choroid plexus cells in 96-well microtiter plates. Several of these clones produce HIV-1 virus mutants that accumulate the gag precursor...
Topics
- Animals
- Blotting, Western
- Cell Line
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Viral
- Gene Products, gag
- Genes, gag
- HIV Envelope Protein gp120
- HIV-1
- Humans
- Microscopy, Electron
- Mutation
- Protein Precursors