Article
Biological characterization of infectious molecular clones derived from a human immunodeficiency virus type-1 isolate with rapid/high replicative capacity.
Virology - 1 Mar 1991
Fredriksson R, Stålhanske P, von Gegerfelt A, Lind B, Aman P, Rassart E, Fenyö E M
Abstract excerpt
In order to molecularly characterize rapidly and slowly replicating HIV-1 variants, molecular clones were obtained from a rapid/high virus isolate. This isolate, 4803, had only been passaged in peripheral blood mononuclear cells (PBMC) prior to cloning. Molecular cloning was done in bacteriophage...
Topics
- Cell Line
- Cells, Cultured
- Cloning, Molecular
- Genetic Variation
- HIV-1
- Humans
- Lymphocytes
- RNA-Directed DNA Polymerase
- Restriction Mapping
- Transfection
- Virus Replication