Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genetic engineering of a Lemna isoleucine auxotroph.

Transgenic research - 1 Oct 2012

Nguyen Long V, Cox Kevin M, Ke John S, Peele Charles G, Dickey Lynn F

Abstract excerpt

Lemna, a member of the Lemnaceae or duckweed family, is a small aquatic plant that can be quickly transformed to produce recombinant proteins in a contained and controlled bioprocessing environment. The containment capability of Lemna has been further improved with the creation of an auxotroph platform that requires isoleucine supplementation for survival of transformed plant lines. Using an RNAi based approach,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Expression Regulation, Enzymologic
Share to Genetic Engineering
Share to Genetic Vectors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hemagglutination Tests

Share to Hemagglutinin Glycoproteins, Influenza Virus

Share to Influenza A Virus, H5N1 Subtype

Share to Isoleucine

Share to Transformation, Genetic

Source

Identifiers

PMID: 22311339
DOI: 10.1007/s11248-012-9594-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genetic engineering of a Lemna isoleucine auxotroph.PMID 22311339Read ↗

Efficient transformation and artificial miRNA gene silencing in Lemna minor.Semantic proximity Read ↗

Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls.Semantic proximity Read ↗

Arabidopsis methionine gamma-lyase is regulated according to isoleucine biosynthesis needs but plays a subordinate role to threonine deaminase.Semantic proximity Read ↗

Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation.Semantic proximity Read ↗

Phenotypic suppression by incorporation of an alien amino acid.Semantic proximity Read ↗

Interdependence of threonine, methionine and isoleucine metabolism in plants: accumulation and transcriptional regulation under abiotic stress.Semantic proximity Read ↗

Identification of the thiamin salvage enzyme thiazole kinase in Arabidopsis and maize.Semantic proximity Read ↗

A site-directed mutagenesis interrogation of the carboxy-terminal end of Arabidopsis thaliana threonine dehydratase/deaminase reveals a synergistic interaction between two effector-binding sites and contributes to the development of a novel selectable marker.Semantic proximity Read ↗

Conquering isoleucine auxotrophy of Escherichia coli BLR(DE3) to recombinantly produce spider silk proteins in minimal media.Semantic proximity Read ↗

Translation start sequences affect the efficiency of silencing of Agrobacterium tumefaciens T-DNA oncogenes.Semantic proximity Read ↗

Biochemical and mass spectrometric evidence for quaternary structure modifications of plant threonine deaminase induced by isoleucine.Semantic proximity Read ↗

Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.