Article
Isolating the Arabidopsis thaliana genes for de novo purine synthesis by suppression of Escherichia coli mutants. I. 5'-Phosphoribosyl-5-aminoimidazole synthetase.
Plant physiology - 1 Jun 1993
Senecoff J F, Meagher R B
Abstract excerpt
We have initiated an investigation of the de novo purine nucleotide biosynthetic pathway in the plant Arabidopsis thaliana. Functional suppression of Escherichia coli auxotrophs allowed the direct isolation of expressed Arabidopsis leaf cDNAs. Using this approach we have successfully suppressed m...
Topics
- Amino Acid Sequence
- Animals
- Arabidopsis
- Base Sequence
- Carbon-Nitrogen Ligases
- Cloning, Molecular
- DNA, Complementary
- Escherichia coli
- Gene Expression Regulation
- Genes, Plant
- Humans
- Ligases
- Molecular Sequence Data
- Mutation
- Phylogeny
- Plants
- Purine Nucleotides
- Sequence Homology, Amino Acid