Article
Optimized virus-induced gene silencing in Solanum nigrum reveals the defensive function of leucine aminopeptidase against herbivores and the shortcomings of empty vector controls.
The New phytologist - 1 Jul 2008
Hartl Markus, Merker Holger, Schmidt Dominik D, Baldwin Ian T
Abstract excerpt
Virus-induced gene silencing (VIGS) enables high-throughput analysis of gene function in plants but is not universally applicable and requires optimization for each species. Here a VIGS system is described for Solanum nigrum, a wild relative of tomato and potato and a valuable model species for ecogenomics. The efficiency of the two most widely used Tobacco rattle virus (TRV) vectors to silence phytoene...
Topics
- Animals
- Feeding Behavior
- Gene Expression Regulation, Plant
- Gene Silencing
- Genotype
- Larva
- Leucyl Aminopeptidase
- Manduca
- Plant Leaves
- Plant Proteins
- Plant Viruses