Article
Evaluation of the Escherichia coli threonine deaminase gene as a selectable marker for plant transformation.
Planta - 1 Mar 2004
Ebmeier A, Allison L, Cerutti H, Clemente T
Abstract excerpt
The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to alpha-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. Previous reports have shown that in planta expression of the wild-type Escherichia coli TD, ilvA,...
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