Article
Kinetics of α-globin binding to α-hemoglobin stabilizing protein (AHSP) indicate preferential stabilization of hemichrome folding intermediate.
The Journal of biological chemistry - 30 Mar 2012
Mollan Todd L, Khandros Eugene, Weiss Mitchell J, Olson John S
Abstract excerpt
Human α-hemoglobin stabilizing protein (AHSP) is a conserved mammalian erythroid protein that facilitates the production of Hemoglobin A by stabilizing free α-globin. AHSP rapidly binds to ferrous α with association (k'(AHSP)) and dissociation (k(AHSP)) rate constants of ≈10 μm(-1) s(-1) and 0.2 s(-1), respectively, at pH 7.4 at 22 °C. A small slow phase was observed when AHSP binds to excess ferrous αCO. This...
Topics
- Blood Proteins
- Hemeproteins
- Hemoglobin A
- Humans
- Kinetics
- Models, Molecular
- Molecular Chaperones
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Protein Binding