Article
[Attenuated Salmonella typhimurium as DNA delivery vehicle for DNA-mediated immunization].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Sept 2004
He Ping, Chen Hong, Liu Jing-xing, Chen Shu-yun, Hu Bao-yu
Abstract excerpt
OBJECTIVE: To study whether the live attenuated AroA-auxotrophic mutant of Salmonella (S.) typhimurium (SL7207) could be used as DNA delivery vehicle to induce more efficient immune response by using the eukaryotic expression plasmid pCMV-beta as report gene. METHODS: Murine peritoneal macrophages were infected with SL7207(pCMV-beta) in vitro, then the expression of the beta-gal were detected by X-gal staining or...
Topics
- Animals
- Bacterial Vaccines
- Cell Proliferation
- Cytotoxicity, Immunologic
- Female
- Genes, Reporter
- Genetic Vectors
- Macrophages, Peritoneal
- Mice
- Mice, Inbred BALB C
- Mutation
- Salmonella typhimurium
- T-Lymphocytes, Cytotoxic