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Article

Retrieval of glycoside hydrolase family 9 cellulase genes from environmental DNA by metagenomic gene specific multi-primer PCR.

Biotechnology letters - 1 May 2012

Xiong Xiaolong, Yin Xiaopu, Pei Xiaolin, Jin Peng, Zhang Ao, Li Yan, Gong Weibo, Wang Qiuyan

Abstract excerpt

A new method, termed metagenomic gene specific multi-primer PCR (MGSM-PCR), is presented that uses multiple gene specific primers derived from an isolated gene from a constructed metagenomic library rather than degenerate primers designed based on a known enzyme family. The utility of MGSM-PCR was shown by applying it to search for homologues of the glycoside hydrolase family 9 cellulase in metagenomic DNA. The...

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PMID: 22261869
DOI: 10.1007/s10529-012-0855-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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