Article
Chitinase gene sequences retrieved from diverse aquatic habitats reveal environment-specific distributions.
Applied and environmental microbiology - 1 Dec 2004
LeCleir Gary R, Buchan Alison, Hollibaugh James T
Abstract excerpt
Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presumptive chitinase genes were created for nine water and six sediment samples from 10 aquatic...
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