Article
Metatranscriptomics of Soil Eukaryotic Communities.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Yadav Rajiv K, Bragalini Claudia, Fraissinet-Tachet Laurence, Marmeisse Roland, Luis Patricia
Abstract excerpt
Functions expressed by eukaryotic organisms in soil can be specifically studied by analyzing the pool of eukaryotic-specific polyadenylated mRNA directly extracted from environmental samples. In this chapter, we describe two alternative protocols for the extraction of high-quality RNA from soil samples. Total soil RNA or mRNA can be converted to cDNA for direct high-throughput sequencing. Polyadenylated...
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