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Article

Analysis of bacterial xylose isomerase gene diversity using gene-targeted metagenomics.

Journal of bioscience and bioengineering - 1 Aug 2015

Nurdiani Dini, Ito Michihiro, Maruyama Toru, Terahara Takeshi, Mori Tetsushi, Ugawa Shin, Takeyama Haruko

Abstract excerpt

Bacterial xylose isomerases (XI) are promising resources for efficient biofuel production from xylose in lignocellulosic biomass. Here, we investigated xylose isomerase gene (xylA) diversity in three soil metagenomes differing in plant vegetation and geographical location, using an amplicon pyrosequencing approach and two newly-designed primer sets. A total of 158,555 reads from three metagenomic DNA replicates...

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PMID: 25656071
DOI: 10.1016/j.jbiosc.2014.12.022

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