Article
[Expression, purification and interaction of human leukocyte antigen F and cluster of differentiation 8alpha homodimers].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Oct 2011
Luo Kaihua, Fan Zheng, Li Hongbin, Liu Yiwei
Abstract excerpt
To obtain large quantity of human leukocyte antigen F (HLA-F) and cluster of differentiation 8alpha homodimers (CD8alphaalpha) proteins and to study their relationship, HLA-F and CD8alpha genes with rare codon in Escherichia coli were cloned using an N-terminal synonymous mutation method. High-efficiency expression protein inclusion bodies were acquired. The proteins were refolded using the dilution method and...
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