Article
[Expression of Bm-TFF2 mutants in Escherichia coli and their cell migration-promoting activity].
Dong wu xue yan jiu = Zoological research - 1 Aug 2011
Yu Guo-Yu, Xiang Yang, Zhang Hong-Yun, Jiang Ping, Lee Wen-Hui, Zhang Yun, Zhang Yong
Abstract excerpt
Bm-TFF2, a trefoil factor from the large-webbed bell toad (Bombina maxima), can stimulate cell migration and inhibit cell apoptosis. To study the structure-function relationship of Bm-TFF2, we constructed wild-type and mutated Bm-TFF2 plasmids and expressed recombinant proteins in E. coli. The wild-type Bm-TFF2 gene encoding mature peptide was obtained by RT-PCR, while the N-terminal, C-terminal and two arginine...
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