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[In vitro expression activity of α-(1,2) fucosyltransferase with 35C > T and 682A > G mutations].

Zhongguo shi yan xue ye xue za zhi - 1 Dec 2010

He Yan-Min, Hong Xiao-Zhen, Ma Kai-Rong, Lan Xiao-Fei, Xu Xian-Guo, Zhu Fa-Ming, Lü Hang-Jun, Yan Li-Xing

Abstract excerpt

In order to explore the effects of 35C > T and 682A > G mutations on the activity of alpha-(1,2) fucosyltransferase, the coding region of fut1 gene was amplified by polymerase chain reaction (PCR) from genomic DNA. PCR product was ligated into expression vector using TOPO TA cloning kit to obtain the recombinant plasmids. The recombinant plasmids were transfected into COS-7 cells by liposome method. After...

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PMID: 21176381

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