Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities.

Abstract excerpt

The microbial world has been shown to hold an unimaginable diversity. The use of rRNA genes and PCR amplification to assess microbial community structure and diversity present biases that need to be analyzed in order to understand the risks involved in those estimates. Herein, we show that PCR amplification of specific sequence targets within a community depends on the fractions that those sequences represent to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to DNA, Bacterial
• Share to Genes, Bacterial