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Article

Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens.

Current genetics - 1 Feb 2012

Sebastianes Fernanda L S, Lacava Paulo T, Fávaro Léia C L, Rodrigues Maria B C, Araújo Welington L, Azevedo João L, Pizzirani-Kleiner Aline A

Abstract excerpt

We describe the genetic transformation of the mycelial tissue of Diaporthe phaseolorum, an endophytic fungus isolated from the mangrove species Laguncularia racemosa, using Agrobacterium tumefaciens-mediated transformation (ATMT). ATMT uses both the hygromycin B resistant (hph) gene and green fluorescent protein as the selection agents. The T-DNA integration into the fungal genome was assessed by both PCR and...

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PMID: 22210192
DOI: 10.1007/s00294-011-0362-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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