Article
Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the fungus Penicillium marneffei.
Mycological research - 1 Aug 2008
Zhang Piyan, Xu Bin, Wang Yuezhu, Li Yunqian, Qian Zhen, Tang Shengrong, Huan Shen, Ren Shuangxi
Abstract excerpt
Penicillium marneffei is an opportunistic fungal pathogen of humans, causing respiratory, skin, and systemic mycosis in south-east Asia. Here we describe the transformation of P. marneffei with Agrobacterium tumefaciens, and the optimization of the transformation procedure. Transformations in different combinations between A. tumefaciens stains (LBA4404 and EHA105) and binary vectors (pCB309A, pBI129A, and...
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