Article
Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection.
Current genetics - 1 Apr 2001
Daniell H, Muthukumar B, Lee S B
Abstract excerpt
Chloroplast genetic engineering offers several advantages over nuclear transformation including high levels of gene expression and gene containment. However, a consequence of placing a transgene in the chloroplast genome is that the antibiotic resistance genes used as selectable markers are highly amplified. Engineering genetically modified (GM) crops without the use of antibiotic resistance genes should...
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