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Article

Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells.

Chemistry & biology - 27 May 2011

Peck Sun H, Chen Irwin, Liu David R

Abstract excerpt

Laboratory-created small-molecule-dependent inteins enable protein structure and function to be controlled posttranslationally in living cells. Previously we evolved inteins that splice efficiently in Saccharomyces cerevisiae only in the presence of the cell-permeable small molecule 4-hydroxytamoxifen (4-HT). In mammalian cells, however, these inteins exhibited lower splicing efficiencies and slower splicing in...

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Identifiers

PMID: 21609843
DOI: 10.1016/j.chembiol.2011.02.014
PMCID: PMC3124510

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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