Article
Nascent-seq indicates widespread cotranscriptional pre-mRNA splicing in Drosophila.
Genes & development - 1 Dec 2011
Khodor Yevgenia L, Rodriguez Joseph, Abruzzi Katharine C, Tang Chih-Hang Anthony, Marr Michael T, Rosbash Michael
Abstract excerpt
To determine the prevalence of cotranscriptional splicing in Drosophila, we sequenced nascent RNA transcripts from Drosophila S2 cells as well as from Drosophila heads. Eighty-seven percent of the introns assayed manifest >50% cotranscriptional splicing. The remaining 13% are cotranscriptionally spliced poorly or slowly, with ∼3% being almost completely retained in nascent pre-mRNA. Although individual introns...
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