Article
Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae.
Journal of biotechnology - 1 Jan 2012
Suzuki Hiroaki, Imaeda Takao, Kitagawa Takao, Kohda Katsunori
Abstract excerpt
We have estimated the effects of hyper-mannosylation of dockerin-type cellulase on cellulosome assembly by using Saccharomyces cerevisiae and 44 protein glycosylation mutants, because the heterologous protein displayed on yeast is assumed to be modified by yeast-specific hyper-mannosylation. First, we constructed the yeast strain CtminiCipA, which displays a heterologous scaffolding protein (miniCipA from...
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