Article
The allele distribution in next-generation sequencing data sets is accurately described as the result of a stochastic branching process.
Nucleic acids research - 1 Mar 2012
Heinrich Verena, Stange Jens, Dickhaus Thorsten, Imkeller Peter, Krüger Ulrike, Bauer Sebastian, Mundlos Stefan, Robinson Peter N, Hecht Jochen, Krawitz Peter M
Abstract excerpt
With the availability of next-generation sequencing (NGS) technology, it is expected that sequence variants may be called on a genomic scale. Here, we demonstrate that a deeper understanding of the distribution of the variant call frequencies at heterozygous loci in NGS data sets is a prerequisite for sensitive variant detection. We model the crucial steps in an NGS protocol as a stochastic branching process and...
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