Article
[Establishment of molecular beacon real-time PCR for detecting p53 gene single nucleotide polymorphism].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Nov 2011
Liao Nong, Gao Jian-hua, Zeng Wei-sen, Lu Feng, Luo Shen-qiu
Abstract excerpt
OBJECTIVE: To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene. METHODS: Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed...
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