Article
The method of single-nucleotide variations detection using capillary electrophoresis and molecular beacons.
Molecular biology reports - 1 Sept 2009
Wang Jinhui, Wang Wei, Liu Yanhong, Duo Libo, Huang Lijuan, Jiang Xiaofeng
Abstract excerpt
We demonstrate that single-nucleotide variations in a DNA sequence can be detected using capillary electrophoresis (CE) and molecular beacons (MBs). In this method, the region surrounding the site of a nucleotide variation was amplified in a polymerase chain reaction, then hybridize PCR products with each of MBs. The sequences of the PCR products are different at the site of 2,044 in exon of interleukin (IL)-13...
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