Article
[Investigation of p53 gene mutations in keloids using PCR-SSCP].
Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery - 1 Jul 2003
Liu Yong-bo, Gao Jian-hua, Duan Hong-jie, Liu Xiao-jun
Abstract excerpt
OBJECTIVE: To detect gene mutations of p53 gene (exon 4-6) in fibroblasts. METHODS: Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing. RESULTS: Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids. CONCLUSION: Gene mutations...
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