Article
Purification of active E1 alpha 2 beta 2 of Pseudomonas putida branched-chain-oxoacid dehydrogenase.
European journal of biochemistry - 1 Nov 1995
Hester K, Luo J, Burns G, Braswell E H, Sokatch J R
Abstract excerpt
Active E1 component of Pseudomonas putida branched-chain-oxoacid dehydrogenase was purified from P. putida strains carrying pJRS84 which contains bkdR (encoding the transcriptional activator) and bkdA1 and bkdA2 (encoding the alpha and beta subunits). Expression was inducible, however, 45-, 39- a...
Topics
- 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
- Amino Acid Sequence
- Base Sequence
- Enzyme Activation
- Escherichia coli
- Ketone Oxidoreductases
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Plasmids
- Pseudomonas putida
- Sequence Alignment
- Transcriptional Activation