Article
Expression, localization, and functional analysis of polychlorinated biphenyl degradation genes cbpABCD of Pseudomonas putida.
Applied and environmental microbiology - 1 May 1991
Khan A A, Walia S K
Abstract excerpt
Genes of Pseudomonas putida strains that are capable of degrading polychlorinated biphenyls were cloned in the plasmid vector pUC19. The resultant hybrid plasmid, pAW6194, contained cbpABCD genes on a 9.0-kb DNA fragment that was necessary for the catabolism of polychlorinated biphenyls. These genes were further subcloned on an 8.0-kb HindIII fragment of pAW540. Degradation of 3-chlorobiphenyl,...
Topics
- Biodegradation, Environmental
- Chloramphenicol O-Acetyltransferase
- Chromosome Deletion
- Cloning, Molecular
- DNA Transposable Elements
- Dioxygenases
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Mutation
- Operon
