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Article

Genetic analysis of the Pseudomonas aeruginosa PAO high-affinity branched-chain amino acid transport system by use of plasmids carrying the bra genes.

Journal of bacteriology - 1 Oct 1990

Hoshino T, Kose K

Abstract excerpt

About 30 mutants of Pseudomonas aeruginosa PAO defective in the high-affinity branched-chain amino acid transport system (LIV-I) were isolated by the selection for resistance to 4-aza-DL-leucine, a toxic leucine analog for LIV-I. All of the mutants were complemented by plasmid pKTH24, harboring the braC gene, which encodes the branched-chain amino acid-binding protein, and the four open reading frames named braD,...

Topics

Alleles
Amino Acids, Branched-Chain
Biological Transport
Carrier Proteins
Genes, Bacterial
Genetic Complementation Test
Genotype
Mutation
Plasmids
Pseudomonas aeruginosa
Restriction Mapping

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Identifiers

PMID: 2120184
DOI: 10.1128/jb.172.10.5540-5543.1990
PMCID: PMC526864

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2120184-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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