Article
Identification and analysis of the genes coding for the putative pyruvate dehydrogenase enzyme complex in Acholeplasma laidlawii.
Journal of bacteriology - 1 Feb 1992
Wallbrandt P, Tegman V, Jonsson B H, Wieslander A
Abstract excerpt
A monospecific antibody recognizing two membrane proteins in Acholeplasma laidlawii identified a plasmid clone from a genomic library. The nucleotide sequence of the 4.6-kbp insert contained four sequential genes coding for proteins of 39 kDa (E1 alpha, N terminus not cloned), 36 kDa (E1 beta), 5...
Topics
- Acholeplasma laidlawii
- Amino Acid Oxidoreductases
- Amino Acid Sequence
- Base Sequence
- Chromatography, Gel
- Cloning, Molecular
- Genetic Complementation Test
- Humans
- Immunoblotting
- Leucine Dehydrogenase
- Molecular Sequence Data
- Mutation
- Plasmids
- Pyruvate Dehydrogenase Complex