Article
Improved methods for genotype determination of human alcohol dehydrogenase (ADH) at ADH 2 and ADH 3 loci by using polymerase chain reaction-directed mutagenesis.
Clinical chemistry - 1 Oct 1990
Groppi A, Begueret J, Iron A
Abstract excerpt
The human gene for producing alcohol dehydrogenase (ADH; EC 1.1.1.1) is polymorphic at ADH 2 and ADH 3 loci. Until now, the study of this polymorphism required liver biopsy or allele-specific radioactive probes. We have used directed mutagenesis by the polymerase chain reaction (PCR) to amplify and analyze the genotype of ADH 2 and ADH 3 loci. Thus, we could determine easily and unambiguously the complete...
Topics
- Alcohol Dehydrogenase
- Alleles
- Base Sequence
- Binding Sites
- Genotype
- Humans
- Isoenzymes
- Molecular Sequence Data
- Mutagenesis
- Polymerase Chain Reaction