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Article

Structural studies on aldolase isozymes through protein engineering.

Progress in clinical and biological research - 1 Jan 1990

Takasaki Y, Kitajima Y, Takahashi I, Sakakibara M, Mukai T, Hori K

Abstract excerpt

Enzymatic studies on aldolase isozymes have been carried out by techniques of protein engineering. Site-directed mutagenesis helps us to verify the roles of amino acid residues in catalytic reactions. Chimeric fusion proteins give us information about the regions which specify the characteristics of the isozymes. The results are: (1) In aldolase A, COOH terminal Tyr and Lys-107 residues play important roles in...

Topics

Amino Acid Sequence
Anemia, Hemolytic
Animals
Chimera
Cloning, Molecular
Escherichia coli
Fructose-Bisphosphate Aldolase
Genetic Engineering
Humans
Isoenzymes
Models, Molecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Protein Conformation

Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 2203067

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2203067-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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