Human aldolase C: characterization of the recombinant enzyme expressed in Escherichia coli.

doi:10.1093/oxfordjournals.jbchem.a124475

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Article

Human aldolase C: characterization of the recombinant enzyme expressed in Escherichia coli.

Journal of biochemistry - 1 Jun 1994

Kusakabe T, Motoki K, Hori K

Abstract excerpt

To study the structure/function relationship and enzymatic properties of human aldolase C, we have constructed an Escherichia coli expression plasmid, pHAC11, for the isozyme. E. coli cells carrying this plasmid produced enzymatically active human aldolase C. The kcat and Km values for fructose-1...

Topics

Base Sequence
Cloning, Molecular
DNA, Complementary
Escherichia coli
Fructose-Bisphosphate Aldolase
Humans
Isoenzymes
Molecular Sequence Data
Mutation
Open Reading Frames
Plasmids
Polymerase Chain Reaction
Recombinant Proteins
Structure-Activity Relationship

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