Article
Alternative methods to a TaqMan assay to detect a tri-allelic single nucleotide polymorphism rs757210 in the HNF1β gene.
Clinical chemistry and laboratory medicine - 21 Oct 2011
Swen Jesse J, Baak-Pablo Renee F, Guchelaar Henk-Jan, van der Straaten Tahar
Abstract excerpt
BACKGROUND: Several studies report difficulties in genotyping HNF1β rs757210 using TaqMan probes. This is possibly due to the tri-allelic nature of this single nucleotide polymorphism (SNP). The aim of the present research was to develop alternative methods for genotyping rs757210. METHODS: Pyrosequencing and high resolution melting analysis of small amplicons (HRM) were developed and tested in panels of type 2...
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