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Homogeneous scoring of single-nucleotide polymorphisms: comparison of the 5'-nuclease TaqMan assay and Molecular Beacon probes.

BioTechniques - 1 Apr 2000

Täpp I, Malmberg L, Rennel E, Wik M, Syvänen A C

Abstract excerpt

Homogeneous assays based on real-time fluorescence monitoring during PCR are relevant alternatives for large-scale genotyping of single-nucleotide polymorphisms (SNPs). We compared the performance of the homogeneous TaqMan 5'-nuclease assay and the Molecular Beacon assay using three SNPs in the h...

Topics

Alleles
DNA
DNA Primers
DNA Probes
Evaluation Studies as Topic
Genetic Testing
Genotype
Heterozygote
Homozygote
Humans
Molecular Probe Techniques
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Single Nucleotide

Receptors, Estrogen

Sensitivity and Specificity

Taq Polymerase

Source

Identifiers

PMID: 10769752
DOI: 10.2144/00284rr02

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026. Prepared record publication-10769752-2026-08-31.1. Manifest publication-graph-cohort-2026-08-31.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 31 Aug 2026.

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