Article
Deletion analysis of the human insulin receptor ectodomain reveals independently folded soluble subdomains and insulin binding by a monomeric alpha-subunit.
The Journal of biological chemistry - 5 Aug 1990
Schaefer E M, Siddle K, Ellis L
Abstract excerpt
A series of 13 deletions within the extracellular domain of the human insulin receptor delineates the boundaries of subdomains that fold de novo into stable proteins that are efficiently secreted and retain the epitopes required for interaction with two conformation-specific monoclonal antibodies. While most of these proteins fail to bind insulin, a truncation that includes only the alpha-subunit is secreted as a...
Topics
- Animals
- Binding Sites
- Binding, Competitive
- Cell Line
- Chromatography, Gel
- Chromosome Deletion
- Cloning, Molecular
- DNA
- Escherichia coli
- Humans
- Insulin