Article
Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.
FEBS letters - 4 Jun 1990
Nakahigashi K, Inokuchi H, Ozeki H
Abstract excerpt
The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both o...
Topics
- Base Composition
- Base Sequence
- Chloroplasts
- Cloning, Molecular
- Escherichia coli
- Exons
- Gene Expression
- Genes, Plant
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotide Probes
- Plants
- RNA, Transfer, Amino Acid-Specific
- RNA, Transfer, Lys
- Suppression, Genetic