Article
The effect of N-terminal changes on arginyl-tRNA synthetase from Escherichia coli.
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica - 1 Mar 2002
Liu Wen, Liu Mo-Fang, Xia Xia, Wang En-Duo, Wang Ying-Lai
Abstract excerpt
An Asn(2) deleted mutant of Escherichia coli arginyl-tRNA synthetase deleted Asn(2) and a chimera mutant, in which the N-terminal 23 amino acid residues of yeast arginyl-tRNA synthetase were appended to the N-terminus of Escherichia coli synthetase, were synthesized and studied. The expression of the deletion and chimera mutants in Escherichia coli formed inclusion bodies, presumably due to improper folding of...
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