Article
Acyl-CoA subunit selectivity in the pikromycin polyketide synthase PikAIV: steady-state kinetics and active-site occupancy analysis by FTICR-MS.
Chemistry & biology - 23 Sept 2011
Bonnett Shilah A, Rath Christopher M, Shareef Abdur-Rafay, Joels Joanna R, Chemler Joseph A, Håkansson Kristina, Reynolds Kevin, Sherman David H
Abstract excerpt
Polyketide natural products generated by type I modular polyketide synthases (PKSs) are vital components in our drug repertoire. To reprogram these biosynthetic assembly lines, we must first understand the steps that occur within the modular "black boxes." Herein, key steps of acyl-CoA extender unit selection are explored by in vitro biochemical analysis of the PikAIV PKS model system. Two complementary...
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