Article
Expression and secretion of Mirabilis antiviral protein in Escherichia coli and its inhibition of in vitro eukaryotic and prokaryotic protein synthesis.
The Journal of biological chemistry - 5 Jul 1990
Habuka N, Akiyama K, Tsuge H, Miyano M, Matsumoto T, Noma M
Abstract excerpt
Mirabilis antiviral protein (MAP), a ribosome-inactivating protein, exhibits inhibitory effects on both plant virus infection and protein synthesis. To study these functions by site-specific mutagenesis, the total synthetic gene of MAP was constructed and expressed in Escherichia coli. However, t...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- N-Glycosyl Hydrolases
- Plant Proteins
- Plasmids
- Promoter Regions, Genetic
- Protein Biosynthesis
- Protein Sorting Signals
- Protein Synthesis Inhibitors
- Ribosome Inactivating Proteins, Type 1