Article
The expression of N-terminal deletion DNA pilot proteins inhibits the early stages of phiX174 replication.
Journal of virology - 1 Oct 2009
Ruboyianes Mark V, Chen Min, Dubrava Mathew S, Cherwa James E, Fane Bentley A
Abstract excerpt
The phiX174 DNA pilot protein H contains four predicted C-terminal coiled-coil domains. The region of the gene encoding these structures was cloned, expressed in vivo, and found to strongly inhibit wild-type replication. DNA and protein synthesis was investigated in the absence of de novo H protein synthesis and in wild-type-infected cells expressing the inhibitory proteins (DeltaH). The expression of the DeltaH...
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